Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

What are the recommended protocols for in situ hybridization?

April 26, 2017hybridization protocols recommended situ

0

Posted

What are the recommended protocols for in situ hybridization?

1 Answer

0

Posted

As for immunohistochemistry, we have no special protocols. General hydration recommendations for immunohistochemistry apply. We have used colorimetric, avitin-biotin complex methods with good results for U6, a ubiquitously expressed mRNA, and EBNA1, an EBV transcript. Others have had excellent results with radioactive methods.