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What can naked DNA still show the world of biology 50 years after Watson and Crick?

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What can naked DNA still show the world of biology 50 years after Watson and Crick?

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The structure of DNA is very well described and thought by most biologists to be quite well understood, at least in principle. But not all aspects of DNA have been very well described. For example, most of the information we have about separating DNA strands comes from thermal denaturation and short oligo studies. As it turns out thermal denaturation is not the natural manner by which organisms usual separate their strands. It is becoming clear that DNA doesn’t behave as an entirely predictable heteropolymer when comparing experimental results with physics models either (see references below). Most importantly, at 39 deg C the force required to separate double stranded DNA becomes less than half that which it is at 35 deg C. This difference may have significant effects on the energy required to replicate or to transcribe depending on temperature. It may also offer an explanation to the advantage of being warmblooded. In order to investigate via which mechanisms DNA achieves this reduct

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