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What causes the xylene (or xylene substitute) dehydration solutions to turn cloudy when a slide from the previous alcohol dish is moved forward into the xylene dish?

April 26, 2017alcohol causes cloudy dehydration previous slide solutions turn xylene

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What causes the xylene (or xylene substitute) dehydration solutions to turn cloudy when a slide from the previous alcohol dish is moved forward into the xylene dish?

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If there is too much water carryover from the last alcohol dish, the xylene solution may turn cloudy. When this occurs, replace the 100% alcohol dishes, back up the slide into 70% alcohol (you can also use a series of steps – 95%, then 70%), allow it to stand for 15 min and then move the slide forward through the 100% alcohol steps and xylene steps.