What factors may contribute to high backgrounds in the Biotrak Eicosanoid assays?
A. If high background is seen only in samples, this suggests some matrix effect is at fault and could relate to a problem either with sample preparation, extraction or dilution of the sample. If replicate OD readings are not within a reasonable %CV (5-10%), then the problem may be more indicative of pipetting or washing errors. If high background is seen with an NSB control, the problem is suggestive of pipetting or washing errors, or contamination of the TMB substrate. Careful comparison versus a blank will help narrow the possible cause. Click here for more information about Biotrak.
