What happened if the vial is opened?
If the vial is opened, there exists a high possibility that spores of fungus and bacteria will enter the vial and land on the gel, where they will grow satisfactorily thanks to the richness of nutrients that the gel contains. If fungus or bacteria develops on the gel, it will immediately compete with the plant for the nutrients, later destroying the plant. The fungus and bacteria will even feed off the plant itself. It is for this reason that the vial should be opened only at the moment of the transplant.