What if my DNA concentration is less than 5 ng/ul?
We’ve had success with human samples with less DNA (down to 1 ng/ul), but the failure rate increases. We can only guarantee results for complex genomic DNA at 5 ng/ul. For simpler genomes (e.g., yeast) much less DNA is required (as long as the molar level for the genome is comparable to that used for more complex genomes).