What is a good procedure to load minor bases on an ABI 392/394 synthesizer with a minimum loss of amidite inthe priming step?
RESPONSE:When minor base amidites are loaded on an ABI synthesizer using the bottle change procedure ~ 400 l of amidite solution are consumed in the prime step. To minimize this loss a manual procedure can be used to load minor bases with a minimal loss of amidite (~ 200 l). This procedure can be used in the manual control mode on the ABI synthesizer using timed delivery of the reagents to change minor bases. 1. Transfer ~ 3.0 ml anhydrous ACN to a clean amidite bottle. 2. Remove old amidite bottle from the “5” position. Wipe line and replace with amidite bottle containing ACN. 3. Rinse bottle 5 line with ACN and Argon.
