WHAT IS REPRODUCIBILITY BETWEEN INDIVIDUAL SECTORS OF THE SAME CustomArray 4X2K?
CombiMatrix hybridized each 4 sectors of the CustomArray 4X2K with the same RNA target sample, and analyzed raw data without normalization and background correction. As shown in figure 5, all four sectors of each microarray had very similar mean signal intensities, with coefficients of variation (CV) in the range from 4 to 11% (Fig. 5). As shown in Fig. 6, the average pair-wise correlation coefficient (r2) among different sectors on the same microarray was 0.97. When the data were log-transformed, the correlation coefficients r2 between individual sectors further increased to 0.98-0.99. Scatter plots of log-transformed raw signal intensities comparing sectors within the same array are shown in Fig. 7. Practically all data points showed very small changes between individual sectors (please note very tight distribution of data points). CombiMatrix calculated a false positive discovery rate based on 2-fold change between sectors hybridized with the same material. On average, 1 to 3 data p
