What is the antibody yield from ascites fluid?
The yield of monoclonal antibody in ascites fluid depends on two major factors. First, the inherent ability of the hybridoma cell line to synthesize and secrete monoclonal antibody. This inherent monoclonal antibody production rate may be improved with molecular biology intervention. Second, the health, clonality, and environment in which the hybridoma cell line is synthesizing and secreting monoclonal antibody contribute to the productivity of the cell line. The full inherent potential of the cell line to produce monoclonal antibody may be compromised if the culture viability is poor, if the cell line is allowed to exhaust critical media components or if the clonality of the cell line is low. SDIX’s mean antibody yield from ascites production using hundreds of cell lines over the last 2 years is 18.9 mgs of antibody per mouse. The mean has a wide variance due to inherent different among cell lines to synthesize and secrete monoclonal antibody. Some cell lines have produced less than 1
