What is the antibody yield from roller bottle supernatant?
The yield of monoclonal antibody also depends on the inherent ability of the cell line to synthesize and secrete monoclonal antibody and the health, clonality and environment in which the hybridoma cells are synthesizing and secreting monoclonal antibody. Changes in media formulation may improve the antibody yield. SDIX means antibody yield from roller bottle supernatant is 35 mgs per liter. This mean has a large variance due to inherent differences among cell lines to synthesize and secrete monoclonal antibody and due to the improvements in yield realized by manipulating the media formulation. Some cell lines produce less than 10 mgs of antibody per liter while others produce over 50 mgs per liter.
