What is the difference between a Western blot performed under reducing versus non-reducing conditions?
To perform a Western blot under reducing conditions simply include a reducing agent such as dithiothreitol (DTT) or β-mercaptoethanol in the SDS sample buffer. These reducing agents act to disrupt any disulfide bonds. The 150 kDa IgG molecule, for example, will be separated into 2x heavy (~50 kDa) and 2x light chains (~25 kDa).
- What is the difference between a short-range transmitter with an air core versus a short-range transmitter with a ferrite core?
- What is the difference between a Western blot performed under reducing versus non-reducing conditions?
- What’s the difference between Unofficial versus Official Ratings and Results?