What is the difference between the 5S reference assay included with the SYBR Green master mix kit and the 5S rRNA endogenous control primer set?
The 5S reference assay included in the miRCURY LNA™ SYBR Green master mix is based on reverse transcription using the random hexamer primer supplied with the miRCURY LNA™ first strand cDNA synthesis kit. The 5S Endogenous control primer set is based on a gene specific reverse transcription step followed by PCR amplification with specific forward and reverse primers. The 5S reference assay is mainly a positive control assay to check that the components of the system are working properly. The 5S Endogenous control has been developed and validated for use in normalizing microRNA expression experiments.
