What is the difference between the IgY fraction products (Cat No. 01-###) and the Affinity purified products (02-###)?
01-### products are IgY fractions that are purified from chicken egg yolk in a series a precipitation steps. The specific antibody concentration varies between products mainly due to differences in the immunogenity of the antigen. This concentration range from approximately 1 to 3 mg/mL, but there is also unspecific IgY in the preparation. 02-### products are from the same hens as for the corresponding 01-### product. We have started out with an IgY fraction that is purified on a column coupled with the antigen of interest. The 01-### antibodies are applied to the column and unbound antibodies are washed away, before the antibodies are eluted at low pH. The specific antibody is approximately 1 mg/ml but unspecific antibodies have been eliminated. These products are sometimes referred to as monospecific polyclonal antibodies. Thus the specific antibody concentration can be the same for both products, but the affinity-purified product has a higher proportion of specific antibodies. This
