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What is the DNA concentration going into the amplification step?

April 26, 2017amplification concentration dna going step

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What is the DNA concentration going into the amplification step?

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Cluster generation requires very low amounts of DNA libraries. A final concentration of 1-8 pM provides the optimum cluster densities. The method of DNA quantitation is important. Calibrating cluster density with DNA concentration may be determined by running a titration flow cell.

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Cluster generation requires very low amounts of DNA libraries. A final concentration of 10–13 pM provides the optimum cluster densities using the Illumina TruSeq Cluster Kit v3. The method of DNA quantitation is important. Calibrating cluster density with DNA concentration may be determined by running a titration flow cell.