What is the exact nature of the controls for the NCode™ Multi-Species miRNA Microarray ?
Dye normalization control: Alexa Fluor® Dye Control Probes are printed in a range of concentrations on the V2 array to allow for normalization of differences in fluorescent signal intensities between the two capture reagents. Refer to the NCode™ Multi-Species miRNA Microarray Kit V2 User Manual for more detail. The NCode™ Human miRNA Microarray also cotains controls for dye normalization. For the NCode™ Rapid miRNA Labeling System, the NCode™ Dye Normalization Control is a synthetic oligonucleotide that is similar to the oligo(dT) bridge in the ligation mixes. However, instead of bases complementary to the tailed RNA, it includes bases complementary to the Alexa Fluor® Dye Control Probes printed on the NCode™ Multi-Species miRNA Microarray. It is designed to bind the dye-labeled DNA polymers in the ligation mixes to the Dye Control Probes, to normalize for differences in fluorescent signal intensities between the two Alexa Fluor® dyes. Positive Control:The NCode™ Multi-Species miRNA Mi
