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What is the highest cell density obtainable from commonly-used bacterial E.coli growth media?

April 26, 2017bacterial CELL commonly-used density e.coli growth Highest media obtainable

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What is the highest cell density obtainable from commonly-used bacterial E.coli growth media?

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TB can support the highest E.coli cell density when Mg2+ is added and agitation is increased. The highest cell density may be obtained from TB is about OD600 = 18. Some cell strains may reach OD600 = 20, but this result is often inconsistent and cells lose protein production ability at this density or higher.