What is the likelihood, that antibodies generated against synthetic peptides will be able to efficiently recognize the intact protein?
Please be aware that the peptide antigen provided by you or designed and synthesized by us is different from the proteins, as it only represents parts of it. While computational simulations for identification and selection of the most efficient immunogenic peptides show a very high success rate in terms of recognition of the native/intact protein, there is a 10-15% likelihood, that the antibodies generated through the use of synthetic peptides may not recognize the intact “original” protein from which the peptide(s) has/have been derived. This could be observed particularly in immunoprecipitation or immunohistochemistry experiments.
Related Questions
- What happens if I or one of my employees injures a registered finger and the fingerprint scanner was no longer able to recognize the original finger registration?
- Can MicroConstants develop our immunoassay if we do not have antibodies for our protein/peptide?
- What should I consider before ordering synthetic peptide-carrier protein (KLH, BSA) conjugates?