What is the molecular, electronic, and magnetic structure of enzyme active sites?
A good example of this kind of problem is the structure of the oxygen-evolving complex of photosystem II. Based on their EXAFS work and other information, Melvin Klein and coworkers have proposed a model for this structure. K-edge EXAFS is valuable for defining the metal-neighbor distances. Investigating the near-edge x-ray absorption fine structure (NEXAFS) in the chlorine and manganese K-edge region can yield important information about the electronic structure of this complex. • How does the concentration and chemical speciation of elements change across an organism? Living systems are not homogeneous: The gradients of metals and other elements across an organism reveal important information about structure and function. A great deal has already been done by fluorescence microscopy, including, for example, many beautiful studies of calcium waves in different organisms. However, x-ray spectromicroscopy can discover information inaccessible by other means. For example, James Penner-Ha
- Should the electronic grant application include active links to websites that demonstrate proposed grant activities, or should a hard copy be submitted along with a CD?
- How should ongoing sampling and reporting of existing monitoring wells be handled at sites where active remediation is taking place?
- What is the molecular, electronic, and magnetic structure of enzyme active sites?