What is the MTT Assay and what is it used for?
The MTT Assay is a very accurate end point (method) used to measure the cell viability of in vitro human tissue equivalents. The assay is a colorimetric assay that measures the reduction of a tetrazolium component (MTT) into an insoluble formazan product by the mitochondria of viable cells. After incubation of the cells with the MTT reagent for several hours, a solution is added to lyse the cells and solubilize the colored crystals. Samples are read using an ELISA plate reader at a wavelength of 570 nm. The amount of color produced is directly proportional to the number of viable cells.
