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What is the positive control and reference for the ELISA titer test?

April 26, 2017CONTROL ELISA positive Reference Test titer

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What is the positive control and reference for the ELISA titer test?

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A GST protein and rabbit anti-GST antibody pair is used as a positive control and reference in the ELISA titer test. A fixed amount of GST protein is coated onto separate wells on the same ELISA plate used for the antiserum titer test. Four-fold serial dilutions of anti-GST antibody, starting from 1:250 to 1:256,000, are examined for binding activity to the coated GST in the ELISA test.