Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

What is the potential for generation of replication competent virus from vectors by recombination?

0
Posted

What is the potential for generation of replication competent virus from vectors by recombination?

0

Recombination may occur (1) between endogenous murine retroviral sequences in the packaging cells line and (2) between vector coding sequences and the helper packaging sequences to generate infectious viral particles. • Envelope protein in the packaging system: The packaging cell line determines if the vector is ecotropic, xenotropic or amphotropic. • General containment considerations: • BL-1 is appropriate when ecotropic or the native envelope is used and it has been shown to be replication incompetent. BL-2 is appropriate for many constructs, while higher levels may be required depending upon the construct. During production phase of the vector, BL-2 is appropriate until replication incompetence is demonstrated. • Animal Research Considerations: • Housing and husbandry: ABL-1 Contact with feces or urine from infected animals for 72 hours post infection. Contact with tissues and body fluids of infected animals.

Thanksgiving questions

*Sadly, we had to bring back ads too. Hopefully more targeted.