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What is the proper method for visualizing the GEMs with cells using microscope?

April 26, 2017cells gems method microscope visualizing

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What is the proper method for visualizing the GEMs with cells using microscope?

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Cells can be imaged both live and fixed on the GEM. The GEM-cell complex may be viewed using a light microscope. The GEM will appear as a large round sphere with grayish-brown particulates inside. These particles are the silicated iron particles. The cells will be transparent circles on the outside of the GEM sphere. At 20X-40X you should be able to see cells attached to GEM. You may stain the cells with Hoechst or a membrane marker to see individual cells. You may use standard staining techniques to visualize the cells. The CubeMagnet may be used to help manipulate the GEMs into the proper position.