What is the purity of my peptide and how is it measured?
The purity of a peptide is documented in the QC package included with the peptide and is measured by HPLC. This HPLC purity of peptide reflects a ratio (in %) of area of the correct peak versus the sum of areas for all detected peaks at the given UV wavelength (typically 215nm or 220nm). Thus, contaminants not absorbing at these UV wavelengths will remain undetected. Occasionally, peptide impurities may coelute with the major peak in HPLC obscuring the real peptide purity.
