Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

What is the smallest quantity of RNA that can reasonably be measured with the BioPhotometer?

0
Posted

What is the smallest quantity of RNA that can reasonably be measured with the BioPhotometer?

0

The BioPhotometer should not be used to measure an absorbance of less than 0.05 at 260 nm. This OD value corresponds to a RNA concentration of app. 2 µg/ml (= app. 100 ng RNA in 50 µl preparation). Please note that you should measure at 260 nm for a minimum absorbance of 0.1 in order to obtain exact results.

Related Questions

Thanksgiving questions

*Sadly, we had to bring back ads too. Hopefully more targeted.