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What is the smallest quantity of RNA that can reasonably be measured with the BioPhotometer?

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What is the smallest quantity of RNA that can reasonably be measured with the BioPhotometer?

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The BioPhotometer should not be used to measure an absorbance of less than 0.05 at 260 nm. This OD value corresponds to a RNA concentration of app. 2 µg/ml (= app. 100 ng RNA in 50 µl preparation). Please note that you should measure at 260 nm for a minimum absorbance of 0.1 in order to obtain exact results.

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