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What is the transformation efficiency of the lon mutant strains PR745 and ER2508 ?

April 26, 2017efficiency lon mutant strains Transformation

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What is the transformation efficiency of the lon mutant strains PR745 and ER2508 ?

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This strain has a lower transformation efficiency than its parent, PR700. Testing them side by side, PR700 gave about 5 x 10ex6/ug, and PR745 gave ~5 x 10ex5/ug, using cells made competent by the rubidium chloride method. Since the only difference between the strains is the mini-Tn10 in lon, presumably lon-related sickness is the problem, and other protease-deficient strains may be effected as well.