What kinds of research labs use spin concentrators?
10 How do I decide which UF membrane to use? Use the QMWL value closest to, but not greater than, the molecular weight of the protein to be retained and purified. If no other, smaller proteins need to be selectively removed, and protein concentration is greater than the mg/mL level, flow rate may actually be faster using a membrane one QMWL size smaller than the protein molecular weight. This is because proteins just slightly larger than skin pores can tend to settle on pores like marbles on a Chinese Checkerboard. See Membrane Selection Guide for more information. 11 Can I use Apollo to remove larger molecules and expect to quantitatively recover a protein that passes through in the filtrate? This application, known as fractionation, is difficult. Two variables are important to effectively fractionate proteins. The polarization of the larger molecule will tend to increase the retention of the smaller protein. Controlling filtration rate and protein concentration can minimize this effe