What Pressure can be used with Silica-based HPLC columns?
• Silica-based HPLC columns can operate at pressures up to 4000psi, except for anything over 4.6mm ID, then operate at pressures less than 3000psi. Resin-based columns are limited to much lower pressures. Expect a gradual increase in back pressure as the column ages. A sudden increase in pressure usually means that there is a plugged frit at the column inlet. To unplug the frit, reverse the column and flush with an appropriate solvent, with the column disconnected from the detector.
