What protection do amber-colored Eppendorf microcentrifuge tubes provide across the photometric spectrum for light sensitive biological samples?
Amber-colored Eppendorf tubes offer reliable protection from light for nucleic acids or dyes. The following diagram shows a photometric differential measurement of light absorption in the range 240 nm to 700 nm between an amber-colored and a standard Safe-Lock microcentrifuge tube (wall thickness of both tubes: 0.8 mm). In the range 240 nm to 500 nm, the difference in light extinction (DA) between the amber-colored and the standard Safe-Lock microcentrifuge tube is always greater than 2. This means a reduction of light radiation by a factor of 100. At 260 nm (the absorption maximum of nucleic acids), an extinction difference greater than 3 can be obtained, meaning that light radiation is reduced to less than one thousandth. Especially in the case of high-energy, short wavelengths, excellent light protection for sensitive samples is obtained with the amber-colored tubes.
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