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What test should I use in a dog or cat that PREVIOUSLY HAS BEEN SPAYED, but appears to have behavior similar to ESTRUS?

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What test should I use in a dog or cat that PREVIOUSLY HAS BEEN SPAYED, but appears to have behavior similar to ESTRUS?

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DOGS & CATS: LH test: Must be performed when dog or cat is NOT exhibiting signs of heat. A single low LH test (<1 ng/ml) confirms that the bitch or queen is sexually intact. DOGS: There are 3 options depending on where the dog is in the estrous cycle. Vaginal cytology: The vaginal epithelium is a good biologic assay for the presence of estrogen. If the dog is in late proestrus or estrus, one should see > 80% to 90% superficial cells. Serum Progesterone: If the dog had signs of estrus 2 weeks to < 2 months ago, a single serum progesterone concentration may be diagnostic (evidence of luteal structure). HCG stimulation test: Estradiol is measured pre & post HCG administration (250 IU HCG SQ, draw post sample 2 hours after injection; refrigerate samples.) Note: Only choice if exhibiting "heat" at time of sampling. CATS: HCG Stimulation test: Serum progesterone is measured prior to, and 10 days after injection of 250 IU HCG SQ Note: Only choice if exhibiting "heat" at time of sampling.

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