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What test should I use in a dog or cat that previously has been spayed, but appears to have behaviour similar to oestrus?

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What test should I use in a dog or cat that previously has been spayed, but appears to have behaviour similar to oestrus?

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DOGS: There are 3 options depending on where the dog is in the oestrous cycle. • Vaginal cytology: The vaginal epithelium is a good biologic assay for the presence of oestrogen. If the dog is in late pro-oestrus or oestrus, one should see > 60% superficial cells. • Basal Serum Progesterone: If the dog had signs of oestrous 2 weeks to < 2 months ago, a single serum progesterone concentration may be diagnostic (evidence of luteal structure). • HCG stimulation test: Progesterone is measured 1-3 weeks after administration of HCG during behavioural oestrus. Protocol for this test is given in our section on Ovarian remnant syndrome. NB: Progesterone assay must be undertaken on serum samples that have not been collected or stored in gel separation tubes as this will significantly reduce the measured concentration. CATS: HCG stimulation test is also valid for this species. Serum samples for progesterone must not be submitted in gel separation tubes.

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