What type of information can be obtained from an ELISPOT assay?
One key piece of information that can be obtained from ELISPOT assays is the frequency of antigen-specific T-cells within a pool of different cell types. This frequency reflects the clonal size of the antigen-specific T-cells and, therefore, the magnitude of T-cell immunity. Pictures: Example of human IFN-gamma ELISPOT results: 2x10E5 human PBMC/well PBMC stimulated with 0.1 g/ml L.
