What types of purification does Biosearch offer and how do they compare?
Biosearch offers the following purification procedures: Desalting: Every oligo is desalted free of charge to remove residual byproducts from synthesis deprotection and cleavage. However, desalting does not remove truncated sequences RPC (Reverse Phase Cartridge): Cartridge purification provides 80-90% pure (full length) product. This level of purification is recommended for primers or unlabeled oligos. RP-HPLC (Reverse Phase HPLC): Provides ~90% pure (full length) product. Our FAM-BHQ ValuProbes are RP-HPLC purified. Dual HPLC (Reverse Phase and Anion Exchange HPLC): Provides ~97% pure(full length) product and is the method of choice when more stringent purification processes are needed for applications such as multiplexing reactions. Please Note: The purity percentages given are general guidelines and these values can vary depending on the sequence of the oligo.