Whats exciting about enzyme inhibition?
• Potential to tell us about enzyme. • Potential uses as drugs and toxins. • Understanding drugs and toxins to counter etc. Three major types of inhibition: • Competitive inhibition • Noncompetitive inhibition • Uncompetitive inhibition Competitive Inhibition: S & I are mutually exclusive, E can bind to one OR the other. (overhead MvH 11.2) Plots: We can model this inhibition with chemical equations, keeping in mind that S & I are mutually exclusive, E can bind to one OR the other: Model: ; and: ; where . Classically assume binding to same site, but other possibilities also. • steric hindrance between S & I in different sites. • overlapping sites for S & I. • Partial sharing of sites. • Conformational change of enzyme with binding of either such that other can not bind.
