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When I do the PEG transfection, I leave it for 30 min at RT then dilute with W5 buffer over another 30 min. Is it necessary?

April 26, 2017buffer dilute leave min necessary peg RT transfection w5

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When I do the PEG transfection, I leave it for 30 min at RT then dilute with W5 buffer over another 30 min. Is it necessary?

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A11. I have always wondered about the “old” procedure for PEG transfection. The best way to find out is to try it out yourself! When I was streamlining the protocol to make everything simple and fast, I found that 3 min transfection actually worked better than the “standard” 60 min transfection. Find it out yourself how much more time you can actually save!