Which advantage do oligonucleotides have in in situ hybridization?
Oligonucleotides have a number of advantages in in situ hybridization. The most important are (a) precise control of melting temperature (b) avoidance of repetitive sequences, (c) easy and cheap synthesis (d) fast diffusion into the specimen (e) high stability of the probe and (f) – good reproducibility of labelling and hybridization reactions.