Which bacterial strains are available for the Ames II / Ames MPF Mutagenicity Assays?
In the Ames II Salmonella typhimurium TA98 is used for the detection of frameshift mutations and TAMix for the detection of base pair mutations. TAMix is a mixture of the strains TA7001, TA7002, TA7003, TA7004, TA7005, TA7006 in equal proportions. Individually these strains are designed to revert by only one specific base-pair substitution. With this mixture, all the possible base pair substitutions can be represented in one culture. In the Ames MPF series of mutagenicity tests the following S. typhimurium strains recommended by the OECD guideline 471 are available: TA98, TA100, TA1535, and TA1537. Two E.coli strains also mentioned in the guideline 471 are also available in the Ames MPF format: E. coli wp2 uvrA and E. coli wp2 [pKM101].
