Which ES cell lines are offered for gene targeting?
We either offer the F1-hybrid cell line, V6.5, or the C57BL/6N line, JM8A3. Typically, the V6.5 line yields germ line transmission frequencies exceeding 95%. The JM8A3 cell line which is the preferred line used by KOMP offers the advantage to yield mutant mice directly in the inbred C57BL/6 background.