Which quality controls are present in SALSA MLPA kits?
All SALSA MLPA probemixes contain four DNA Quantity control fragments (Q-fragments) and most also contain three DNA Denaturation control fragments (D-fragments). The 4 Q-fragments (64, 70, 76, 82 nt) are not ligation-dependent and are present in small quantities. When the MLPA reaction has been successful, the Q-fragments are dwarfed by the MLPA probes. In case the peaks of all four Q-fragments are larger than half the size of the peaks of the D-fragments and MLPA probes, either the ligation reaction failed or the amount of sample DNA was insufficient. In either case, the MLPA results are not reliable. In contrast, the three D-fragments (88, 92 and 96 nt) are DNA- and ligation-dependent just like normal MLPA probes. Their peak size should be comparable to that of the MLPA probes; they will be visible when ligation was successful and DNA was sufficient and properly denatured. The 92 nt D-fragment recognizes a 2q14 DNA sequence. The 88 nt D-fragment recognizes a sequence on 6p21.3 in the
