Why are my calculated yields lower than the ones provided by Eurofins MWG Operon?
The most common reason for this is that the oligos are not fully dissolved. If you resuspended in water, you may need to add NaOH to raise the pH to between 7 and 8. Oligos resuspended in water tend to have a pH of around 5, and this is often too low for them to completely dissolve. We recommend that most oligos be resuspended in TE at pH 7. We find that 10 mM Tris-HCl, 1 mM EDTA works well, but if your experiment cannot tolerate EDTA, you may use just Tris-HCl.
