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Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?

April 26, 2017assay CT low qPCR qrt-pcr values

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Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?

1 Answer

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You may be using too much template. Use less input total RNA for reverse transcription, or use template at a greater dilution factor (lower concentration). Do not pipet a volume of less than 1 l.