Why are the flow rates for Capture, Purify and Enrich different?
The Capture step requires the flow rate to be slow enough to allow time for the affinity resin to bind the desired protein. However for Purification, the flow rate can be higher since the desired effect is to wash away any unbound proteins. Finally, the Enrichment step can also be performed at higher flow rates because this process involves releasing the desired protein from the affinity resin. (See also Q 13 above).
