Why can some chromosomes have lower confidence calls than others within the same cell?
Confidence levels for one or more chromosome pairs in a cell can vary for a variety of reasons. Sometimes this occurs due to inconsistent DNA amplification, meaning that the DNA from the single cell does not copy equally across all chromosomes. This can occur if the biopsied cell is of poor quality, or if the DNA within the cell is degrading, or sometimes it is just a normal byproduct of the amplification process. The inconsistent DNA amplification can make it difficult to determine whether there are two copies of a chromosome, or just one, or three, leading to a lower confidence call. Lower confidence calls may also occur if both parents have the same genetic marker at a specific data point on a particular chromosome. PGD with Parental Support is based on comparing the genetic blueprints obtained from the parents to the results seen in the embryo. If both parents have the same marker at a specific chromosome location, it is not possible to differentiate the maternal copy from the pate
Confidence levels for one or more chromosome pairs in a cell can vary for a variety of reasons. Sometimes this occurs due to inconsistent DNA amplification, meaning that the DNA from the single cell does not copy equally across all chromosomes. This can occur if the biopsied cell is of poor quality, if the DNA within the cell is degrading, or sometimes it is just a byproduct of the normal amplification process. The inconsistent DNA amplification can make it difficult to determine whether there are two copies of a chromosome, one copy, or three copies, leading to a lower confidence call. Lower confidence calls may also occur if both parents have the same genetic marker at a specific data point on a particular chromosome. PGD with Parental Support is based on comparing the genetic blueprints obtained from the parents to the results seen in the embryo. If both parents have the same marker at a specific chromosome location, it is not possible to differentiate the maternal copy from the pat
