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Why did I get low viral nucleic acid yields from cell culture supernatant when using a QIAamp MinElute Virus kit?

April 26, 2017acid CELL culture low minelute nucleic qiaamp supernatant viral yields

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Why did I get low viral nucleic acid yields from cell culture supernatant when using a QIAamp MinElute Virus kit?

1 Answer

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10 Posted

The QIAamp MinElute Virus Kit contains Protease resuspension buffer which ensures optimal lysis of difficult-to-lyse viruses like HBV. When working with samples containing phosphate buffer we recommen…