Why do some experimental localizations not agree with the previously published localization?
In all cases we recommend reviewing the peptide sequences used, species, cell type, methodology and conditions used to record the localization. In some cases splice variants target to different locations so reviewing the peptide sequence is critical. Also there are many examples of different condtions resulting in different localizations. In all cases we provide the peptide sequence of the clone tested and we have consistently used the same methodology, conditions, transfection time, and reagents to record all localizations. We also provide multiple images for the user to review.
Related Questions
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- Why do some experimental localizations not agree with the previously published localization?
