Why does Campy fail to grow when I try to reconstitute the lyophilized pellets?
A. Campylobacter jejuni is one of the most difficult organisms to revive from a preserved state. Follow the manufacturer’s instructions carefully when reconstituting preserved microorganisms. Never attempt to grow any lyophilized organism in a broth. Always use an appropriate non-selective solid media for growing-up the reconstituted organism. The preferred media to grow Campy on is a Chocolate Agar plate. Once inoculated to the solid media, immediately transfer the culture to the appropriate atmosphere and incubation temperature. Campylobacter spp. must be placed immediately into a jar with a gas generator to create a microaerophilic atmosphere. The jar method is superior to the bag method for generating Campy from a pellet. Allow at least 48 hours at 35 degrees C. for growth; 42 degree C. incubation is not recommended when growing Campy from a preserved state.
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