Why does the control line sometimes appear very weak while the test line is positive?
Control line is aimed to ensure that chromatography has been performed up to the top of the strip. It is not related with the result of the diagnostic itself.Control line may sometimes be “very weak” when the sample is highly positive. Signal on the control line comes from the remaining conjugate that didn’t react with the antigen on the specific live. More positive will be the specific signal, less positive will be the signal on the control line.The control line confirms that migration proceeded successfully even if the line is only slightly rose or dark red.