Why does Upstate have multiple pKD versions for the same gene target? Which one should I buy?
Some journals are requesting that a siRNA derived phenotype be confirmed with more than one siRNA sequence to the same gene. Using multiple siRNAs to the same gene target is a useful control to validate specificity by confirming that a phenotype is the result of targeted gene knock-down and not an off-target, non-specific effect. If the same phenotype results from multiple target-specific pKD siRNA plasmids, the likelihood that the phenotype is due to an off-target event is eliminated.
