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Why is Betaine added to the RiboMinus Hybridization Buffer?

April 26, 2017added betaine buffer hybridization

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Why is Betaine added to the RiboMinus Hybridization Buffer?

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Betaine increases the hybridization stringency. It equalizes the GC Tm and AT Tm, so the background, non-specific, non-rRNA hybridization to the RiboMinus probes due to high GC content may be reduced.