Why is it beneficial to set up crystallization experiments using high-throughput methods, and why use 1536 cocktails?
By using automated liquid-handling systems, we are able to set up crystallization experiments precisely and reproducibly using a minimum volume of macromolecular solutions that are often difficult to obtain. The laboratory personnel start with samples contained in a microcentrifuge tube and, within 10 minutes, are able to set up 1536 unique, microbatch-under-oil crystallization experiments. Setting up the same 1536 experiments manually would take a technician several weeks to complete. This speed is truly advantageous, greatly reducing the time available for sample degradation prior to the crystallization experiment. Finally, by setting up so many chemically diverse crystallization experiments, we increase the likelihood of identifying more than one crystallization condition. Crystals produced from different chemical cocktails will often have different physical properties. The ability to choose from several different initial crystallization conditions provides the researcher with multi
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